Journal: Journal of Veterinary Science

Article Title: Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine

doi: 10.4142/jvs.2013.14.1.53

Figure Lengend Snippet: Percentages of CD3+CD4+ and CD3+CD8+ T lymphocytes in the different groups of chickens. Columns are labeled with letters. Different letters indicate statistically significant differences ( p < 0.01) while letters in common indicated that no significant differences ( p > 0.01) were observed in each T cell subgroup.

Article Snippet: The samples (100 µL; 1 × 10 6 cells) was incubated with mouse anti-chicken CD4-PE (BD Biosciences, USA), mouse anti-chicken CD3-SPRD (BD Biosciences, USA), and mouse anti-chicken CD8-FITC (BD Biosciences, USA) primary antibodies (antibody final concentrations as 1.25 µg/100 µL) in the dark at 4℃ for 30 min. After the incubation, all samples were washed three times with cold PBS and then resuspended in 0.5 mL of PBS.

Techniques: Labeling